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METABOLIC STUDIES OF BILE ACIDS XXI. ‘3β-HYDROXYSTEROL DEHYDROGENASE’ IN RAT LIVER
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- YAMASAKI KAZUMI
- Department of Biochemistry, Tottori University School of Medicine
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- NODA FUMIO
- Department of Biochemistry, Tottori University School of Medicine
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- SHIMIZU KYUTARO
- Department of Biochemistry, Tottori University School of Medicine
Description
1. A partial purification of 3β-hydroxysterol dehydrogenase from rat liver has been described.<br> 2. The enzyme preparation catalyzed the reversible oxidation of 3β-hydroxysterols, regardless of the configuration of the A-B ring junction.<br> 3. DPN+ and TPN+ functioned here equally well as a hydrogen acceptor.<br> 4. The enzyme preparation was inhibited by some sulfhydryl reagents.<br> The authors are indebted to Prof. Dr. L. F. Fieser, Harvard University, for generous gifts of pregnenolone and other steroids. Thanks are due to Miss N. Hatano for her expert technical assistance during the course of this work.
Journal
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- The Journal of Biochemistry
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The Journal of Biochemistry 46 (6), 747-755, 1959
The Japanese Biochemical Society
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Details 詳細情報について
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- CRID
- 1570291228034198656
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- NII Article ID
- 130006877850
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- Text Lang
- en
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- Data Source
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- CiNii Articles