Isolation of calmodulin from the protozoan, Tetrahymena pyriformis, by the use of a tubulin-sepharose 4B affinity column.

J-STAGE
  • KUMAGAI Hiromichi
    Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo
  • NISHIDA Eisuke
    Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo
  • ISHIGURO Koichi
    Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo
  • MUROFUSHI Hiromu
    Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo

この論文をさがす

説明

We developed a simple new method for the isolation of calmodulin, a ubiquitous Ca2+-binding protein, by using tubulin-Sepharose 4 B column chromatography, and succeeded in rapid isolation of calmodulin from Tetrahymena pyriformis. The procedure was also shown to be successfully applicable to the isolation of calmodulins from starfish ovary, porcine brain, and monkey brain and, therefore, may be of general use for the rapid isolation of calmodulin.

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ