Synthesis of Antibody <i>in vitro</i>:I. Immunological Memory Cells Separated from Spleen and Lymph Nodes of the Immunized Rabbits

J-STAGE
  • YUKIOKA Munehiko
    Department of Biochemistry and Department of Physiology, School of Medicine, Osaka City University
  • KINOSHITA Yoshihiro
    Department of Biochemistry and Department of Physiology, School of Medicine, Osaka City University
  • TAKESHITA Tadashi
    Department of Biochemistry and Department of Physiology, School of Medicine, Osaka City University
  • KIMURA Eiichi
    Department of Biochemistry and Department of Physiology, School of Medicine, Osaka City University
  • MORISAWA Seiji
    Department of Biochemistry and Department of Physiology, School of Medicine, Osaka City University

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Description

The lymphoid cells prepared from spleen or lymph nodes of immunized rabbits were fractionated into three groups by multi-layer centrifugation. The most dense cellular population which contained the highest percentage of small lymphocyte showed the specific enhancement of DNA synthesis by the anamnestic antigenic stimulation. During secondary immune response, the immunological memory cells seemed to be converted to a less dense type, and antibody was produced mainly in these less dense cells.<br> Various kinds of antimetabolite of nucleic acid and protein synthesis have shown the remarkable inhibitory effects on antibody formation in vitro system.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 68 (4), 467-473, 1970

    The Japanese Biochemical Society

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