STUDIES ON TAKA-MALTASE:III. INHIBITION OF TAKA-MALTASE I BY CARBOHYDRATES

KATO IKUNOSHIN, MATSUSHIMA TAIJIRO, AKABORI SHIRO

STUDIES ON TAKA-MALTASE:III. INHIBITION OF TAKA-MALTASE I BY CARBOHYDRATES

J-STAGE

KATO IKUNOSHIN

Laboratory of Biochemistry, Faculty of Science, University of Osaka
MATSUSHIMA TAIJIRO

Laboratory of Biochemistry, Faculty of Science, University of Osaka
AKABORI SHIRO

Laboratory of Biochemistry, Faculty of Science, University of Osaka

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説明

Inhibition of Taka-maltase I by carbohydrates was studied, and Michaelis' constants and maximum velocity of the enzymatic action on maltose and α-phenyl-D-glucoside were obtained.<br> Sucrose, raffinose, D-xylose, trehalose and D-glucose were competitive inhibitor, while cellobiose, lactose, D-mannose, D-galactose, rhamnose, mannitol, sorbitol and inositol did not inhibit the enzymatic action. And the affinity of Taka-maltase I for substrates was discussed.<br> The authors wish to express their gratitudes to the Sankyo Go., Ltd. for their kind supply of "Takadiastase Sankyo".