Physicochemical properties of the extracellular hemoglobin from the planorbid snail, Indoplanorbis exustus

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Abstract 1. 1. Physicochemical properties of the extracellular hemoglobin from the planorbid snail Indoplanorbis exustus were investigated. 2. 2. The wavelengths of absorption maxima and millimolar absorption coefficients of the hemoglobin derivatives were determined. Amino acid composition of the hemoglobin was also examined. 3. 3. The native hemoglobin showed a sedimentation constant of 35.5S, and its molecular weight which was determined by sedimentation equilibrium was 2.04 × 10 6 . 4. 4. Iron and heme contents of the hemoglobin were 0.300% and 2.89%, which corresponded to a minimum molecular weight of 18,600 and 21,300, respectively. 5. 5. SDS-PAGE of the hemoglobin gave one main band corresponding to 280 kDa in the unreduced state and one main band corresponding to 170 KDa in the reduced one. 6. 6. The results obtained may indicate that the native hemoglobin is composed of 8 submultiples of 2.8 × 10 5 daltons or 12 polypeptide chains of 1.7 × 10 5 daltons.

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