Cytofluorometry on cells isolated from paraffin sections after blocking of the background fluorescence by azocarmin G

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タイトル別名
  • Cytofluorometry on cells isolated from paraffin sections after blocking of the background fluorescence by Azokarmin G

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説明

A technique for isolation of cells from paraffin embedded tissue is indispensable for the performance of Feulgen-DNA cytofluorometry in parallel with the definition of histological characteristics. Background fluorescence due to nonspecific dye-binding by a "pseudo-plasmal reaction" is usually found to be so intense on cells isolated from formalin-fixed tissues, that we are often forced to abandon quantitative DNA determinations. In the present work, we report the fixation of tissues with Carnoy's fixative for 12 h at 5 degrees C not only reduces nonspecific dye-binding but also facilitates the process of cell isolation. Furthermore, we find that pre-treatment of cells isolated from Carnoy-fixed tissues with acidic azocarmin G solution completely blocks nonspecific dye-binding in subsequent acriflavine Feulgen nuclear staining. This combination of techniques for specimen preparation enables us to carry out Feulgen-DNA cytofluorometry on cells isolated from histological sections with satisfactorily low coefficients of variation (less than 8%). The techniques should be widely applicable for parallel DNA determinations and histology.

収録刊行物

  • Histochemistry

    Histochemistry 71 161-170, 1981

    Springer Science and Business Media LLC

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詳細情報 詳細情報について

  • CRID
    1570572701432194304
  • NII論文ID
    30013138194
  • NII書誌ID
    AA11033302
  • DOI
    10.1007/bf00507820
  • ISSN
    1432119X
    03015564
  • PubMed
    7239943
  • データソース種別
    • CiNii Articles
    • OpenAIRE

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