Effect of the Potential Triplex DNA Region on the In Vivo Expression of Bacterial .BETA.-Lactamase Gene in Superhelical Recombinant Plasmids.

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  • Kato Mikio
    Department of Life Science, College of Integrated Arts and Sciences, University of Osaka Prefecture
  • Shimizu Nobuyoshi
    Department of Molecular Biology, Keio University School of Medicine

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Description

The effect of a pyrimidine/purine-biased stretch which has the potential to form an unusual triplex DNA structure on gene expression has been analyzed by measuring the activity of β-lactamase as a reporter gene in recombinant plasmids. The Escherichia coli transformant carrying the plasmid p7ERS which has a potential triplex DNA region expressed about twofold more β-lactamase activity than that carrying the plasmid pUC19. Since the expression of β-lactamase has been shown to be affected by template topology in vitro, this in vivo observation suggests that the inserted pyrimidine/purine-biased stretch modulates the topology of flanking regions by forming unusual DNA structure to keep the template at the superhelicity favorable for the expression of β-lactamase.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 112 (4), 492-494, 1992

    The Japanese Biochemical Society

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