Type 1 Cu Structure of Blue Nitrite Reductase from Alcaligenes xylosoxidans GIFU 1051 at 2.05 .ANGS. Resolution: Comparison of Blue and Green Nitrite Reductases.

J-STAGE
  • Inoue Tsuyoshi
    Department of Materials Chemistry, Graduate School of Engineering, Osaka University
  • Gotowda Masaharu
    Department of Materials Chemistry, Graduate School of Engineering, Osaka University
  • Deligeer
    Department of Chemistry, Graduate School of Science, Osaka University
  • Kataoka Kunishige
    Department of Chemistry, Graduate School of Science, Osaka University
  • Yamaguchi Kazuya
    Department of Chemistry, Graduate School of Science, Osaka University
  • Suzuki Shinnichiro
    Department of Chemistry, Graduate School of Science, Osaka University
  • Watanabe Hirotaka
    Department of Materials Chemistry, Graduate School of Engineering, Osaka University
  • Gohow Manabu
    Department of Materials Chemistry, Graduate School of Engineering, Osaka University
  • Kai Yasushi
    Department of Materials Chemistry, Graduate School of Engineering, Osaka University

Search this article

Description

The crystal structure of the blue nitrite reductase from Alcaligenes xylosoxidans GIFU 1051 (AxgNIR) has been determined at 2.05 Å resolution. AxgNIR contains both type 1 and 2 Cu sites, the geometry of the former being distorted tetrahedral. The superpositioning of the type 1 Cu sites in the blue enzyme and a green nitrite reductase revealed that the orientation of the Met150 side chain differed. The deviation of the Sδ(Met150) atom from the axial position of the NNS plane formed by two Nδ(His95 and His145) and one Sβ(Cys136) atom caused the difference in the colors of the enzymes, i.e. blue and green.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 124 (5), 876-879, 1998

    The Japanese Biochemical Society

Keywords

Details 詳細情報について

Report a problem

Back to top