Biochemistry of Shellfish Lipid:III. Purification and Elemental Analysis of Ceramide Aminoethylphosphonate from <i>Corbicula</i> Complex Lipid Mixtures

J-STAGE
  • HORI TARO
    Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University
  • ITASAKA OSAMU
    Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University
  • INOUE HIDEO
    Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University

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Description

Purification of ceramide aminoethyl-phosphonate from the corbicula, Corbicula<br>sandai was accomplished by the use of silicic acid column chromatography and by the application of mild alkaline hydrolysis of Dawson. Thus it was found that the pure lipid can be more effectively prepared by the alkaline hydrolysis according to the D a w s o n method than by the silicic acid column chromatography.<br> In addition, some properties and ana-lytical values of the lipid purified by the alkaline hydrolysis method are presented, and they essentially agreed with those estimated for ceramide aminoethylphos-phonate.<br> The authors express their cordial thanks to Prof. T. Yamakawa of the University of Tokyo for his kind advice and encouragement.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 59 (6), 570-573, 1966

    The Japanese Biochemical Society

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