Oncogenic Collaboration of the Cyclin D1 (PRAD1, <i>bcl</i>‐1) Gene with a Mutated p53 and an Activated <i>ras</i> Oncogene in Neoplastic Transformation

DOI Web Site Web Site PubMed 被引用文献1件 参考文献36件 オープンアクセス

この論文をさがす

説明

<jats:p>Cyclin D1 is one of the key regulators in G1 progression in the cell cycle and is also a candidate oncogene (termed PRAD1 or <jats:italic>bcl</jats:italic>‐1) in several types of human tumors. We report a collaboration of the cyclin D1 gene with <jats:italic>ras</jats:italic> and a mutated form of p53 (p53‐mt) in neoplastic transformation. Transfection of cyclin D1 alone or in combination with <jats:italic>ras</jats:italic> or with p53‐mt was not sufficient for focus formation of rat embryonic fibroblasts. However, focus formation induced by co‐transfection of ras and p53‐mt was enhanced in the presence of the cyclin D1‐expression plasmid. Co‐transfection of <jats:italic>ras</jats:italic>‐ and p53‐mt‐transformants with the cyclin D1‐expression plasmid resulted in reduced serum dependency in <jats:italic>vitro</jats:italic>, Furthermore, the transformants expressing exogenous cyclin D1 grew faster than those without the cyclin D1 plasmid when injected into nude mice. These observations strengthen the significance of cyclin D1 overexpression through gene rearrangement or gene amplification observed in human tumors as a step in multistep oncogenesis; deregulated expression of cyclin D1 may reduce the requirement for growth factors and may stimulate in <jats:italic>vivo</jats:italic> growth.</jats:p>

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (36)*注記

もっと見る

キーワード

問題の指摘

ページトップへ