Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.
-
- Y Gavrieli
- Department of Experimental Medicine, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
-
- Y Sherman
- Department of Experimental Medicine, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
-
- S A Ben-Sasson
- Department of Experimental Medicine, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
抄録
<jats:p>Programmed cell death (PCD) plays a key role in developmental biology and in maintenance of the steady state in continuously renewing tissues. Currently, its existence is inferred mainly from gel electrophoresis of a pooled DNA extract as PCD was shown to be associated with DNA fragmentation. Based on this observation, we describe here the development of a method for the in situ visualization of PCD at the single-cell level, while preserving tissue architecture. Conventional histological sections, pretreated with protease, were nick end labeled with biotinylated poly dU, introduced by terminal deoxy-transferase, and then stained using avidin-conjugated peroxidase. The reaction is specific, only nuclei located at positions where PCD is expected are stained. The initial screening includes: small and large intestine, epidermis, lymphoid tissues, ovary, and other organs. A detailed analysis revealed that the process is initiated at the nuclear periphery, it is relatively short (1-3 h from initiation to cell elimination) and that PCD appears in tissues in clusters. The extent of tissue-PCD revealed by this method is considerably greater than apoptosis detected by nuclear morphology, and thus opens the way for a variety of studies.</jats:p>
収録刊行物
-
- The Journal of cell biology
-
The Journal of cell biology 119 (3), 493-501, 1992-11-01
Rockefeller University Press
- Tweet
キーワード
詳細情報
-
- CRID
- 1364233268901089792
-
- NII論文ID
- 80006769241
-
- ISSN
- 15408140
- 00219525
- http://id.crossref.org/issn/00219525
- http://id.crossref.org/issn/00219533
-
- データソース種別
-
- Crossref
- CiNii Articles