Enzymatic synthesis of oligonucleotides of defined sequence. The "single addition" of 2'(3)-O-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase.:The "Single Addition" of 2'(3)-O-Dihydrocinnamoyl-nucleoside 5'-Diphosphate to a Primer Oligonumleotide Catalyzed by a Thermophilic Polynucleotide Phosphorylase

J-STAGE

この論文をさがす

説明

Several oligonucleotides of defined sequence were synthesized using 2'(3')-O-dihydro-cinnamoyl-nucleoside 5'-diphosphates (DHC-NDP) as substrates for polynucleotide phosphorylase [EC 2.7.7.8] from Thermus thermophilus. The enzyme catalyzed the transfer of one nucleotidyl residue from each of the 2'(3')-O-dihydrocinnamoyl esters of CDP, UDP, and GDP to the 3'-terminus of the primer triadenosine diphosphate, (Ap)2A. The products were shown to be (Ap)3C, (Ap)3U, and (Ap)3G by enzymatic analysis.

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ