Structure and fuction of 5 Sribosomal riboncleic acid from Torulopsis utilis.:I. Purification and Complete Digestion with Pancreatic Ribonuclease A and Ribonuclease T<sub>1</sub>

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A large amount of Torulopsis utilis 5S RNA was highly purified by successive chromatography on columns of DEAE-Sephadex A-50 and Sephadex G-100. The purified 5S RNA was completely digested with pancreatic ribonuclease A [EC 3. 1. 4. 22] and ribonuclease T1 [EC 3. 1. 4. 8], and the digestion products were separated and sequenced by column chromatographic procedures. The results of analyses of the two digests were in good agreement, and indicated that this RNA is composed of 121 nucleotide residues and contains no modified nucleotide as the other 5S RNA's so far sequenced do not. This RNA differs in several nucleotides from Saccharomyces carlsbergensis 5S RNA. The complete sequence of this RNA has been established after partial digestion with ribonucleases A, T1, and U2 as described in the subsequent paper.

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