(21)Phylogenetgic Analysis of Ralstonia solanacearum Based on the PCR-Amplified 282 bp Fragment

Villa J.E., Tsuchiya K., Horita M., Opina N.L., Natural M.P., Hyakumachi M.

Strains of Ralstonia solanacearum isolated from different host crops in the Philippines were characterized according to biovar classification and phylogenetic analysis based on the nucleotide sequence alignment of the 282 bp fragment (Opina et al., 1997). Phylogenetic tree showed that the strains were divided into three groups : group 1 consisting of strains belonging to biovars 3 and 4, group 2 consisting of strains belonging to biovars 1 and 2, and group 3 consisting of strains belonging only to biovar 2. The grouping of the strains corresponded to that described by Taghavi et al. (1997) using the sequence of the 16S rRNA. Restriction analysis using a restriction enzyme Nla III for the PCR-amplified 282 bp fragment of 160 strains revealed that all strains belonging to biovars 3 and 4 (group 1) showed restriction fragments of 115 and 167 bp size, strains belonging to biovars 1 and 2 (group 2) showed no restriction, and strains belonging to biovar 2 (group 3) showed restriction fragments of 55 and 227 bp size, respectively, suggesting that RFLP should be useful for quick differentiation of the strains.

(21)Phylogenetgic Analysis of Ralstonia solanacearum Based on the PCR-Amplified 282 bp Fragment

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