Macrophage Activation and Immunostimulating Activity of Sphaerotilus natans and Its Slime Fraction(Biological)

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The macrophage activation and immunostimulating effects of the slime of Sphaerotilus natans IAM 12068, which is an aquatic sheathed bacterium, and GF-P-1, which was isolated from the slime fraction by Sepharose 4B gel filtration and has antitumor activity against Ehrlich ascites tumor in mice, were investigated. C57BL/6 mice peritoneal exudate macrophage induced by GF-P-1, which exhibited more effective antitumor activity than slime fraction, had the strongest cytostatic activity against EL-4 leukemic cells in vitro. Addition of GF-P-1 to mouse peritoneal macrophages in vitro caused marked spreading morphology, but the slime fraction did not. Acid phosphatase activity in the peritoneal cells induced by slime and GF-P-1 was also augmented as compared with that of resident macrophages. S. natans fractions, as well as Salmonella typhimurium lipopoly-saccharide, were able to induce the mitogenic response in cultured spleen cells of C57BL/6 mice. Since spleen cells pretreated with rabbit anti-mouse thymocyte antiserum showed an unaffected mitogenic response to slime, the slime may be a B-lymphocyte mitogen. When slime fraction and GF-P-1 were injected intraperitoneally into ddY mice, they exhibited an enhancing effect on antibody response in vivo. These results indicate that slime and GF-P-1 are able to activate macrophages and exhibit immunostimulating effects.

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