Selective Inhibition of Cholesterol Esterification in High Density Lipoprotein by Triglyceride Hydrohysis of Intralipid or Chylomicron
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- HOMMA Yasuhiko
- Department of Internal Medicine, Tokai University School of Medicine
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- MIKAMI Yoshikazu
- Department of Internal Medicine, Tokai University School of Medicine
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- SHU Kentoku
- Department of Internal Medicine, Tokai University School of Medicine
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- TAMACHI Hiromitsu
- Department of Internal Medicine, Tokai University School of Medicine
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- NAKAYA Noriaki
- Department of Internal Medicine, Tokai University School of Medicine
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- ARAKI Goro
- Department of Internal Medicine, Tokai University School of Medicine
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- GOTO Yuichiro
- Department of Internal Medicine, Tokai University School of Medicine
Bibliographic Information
- Other Title
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- カイロミクロン, VLDL-TG水解過程での, HDLにおけるコレステロールエステル化能の選択的抑制
Abstract
Effects of Intralipid on the increase of esterified cholesterol (EC) in each lipoprotein fraction in the fresh fasting plasma (FP) or triglyceride lipaserich plasma (PHP) from normal, and type V hyperlipoproteinemic subjects were investigated. FP or PHP was mixed with 14C-FC-albumin solution and was preincubated at 4°C for two hours. The mixture was incubated with Intralipid or with 0.15M NaCl solution. Incubation mixtures were collected at 0, 120 and 240 minutes. Chylomicron, VLDL, IDL, LDL and HDL were fractioned ultra-centrifugally at 4°C. Lipids were extracted with chloroform-methanol (2:1) and EC and FC were separated by TLC. The radioactivities of them were measured with a liquid scintillation counter. In the normal FP, Intralipid significantly increased 14C-EC in chylomicron, and VLDL fractions. However, Intralipid reduced cholesterol esterification, and this was solely due to the complete suppression of 14C-EC increase in HDL in normal PHP. The specific activities of EC in HDL were significantly lower than those in LDL in normal PHP with Intralipid. In FP of type V hyperlipoproteinemia, the increase of 14C-EC was more in chylomicron and VLDL fractions and less in LDL and HDL fractions than that in normal FP. The effect of Intralipid addition was very small in FP of type V hyperlipoproteinemia. In PHP of type V hyperlipoproteinemia, the results were very similar to those in normal FP with Intralipid. No increase of 14C-EC was observed in HDL. The effect of Intralipid addision was also small as in FP. Therefore, we concluded as following. Intralipid worked like chylomicron. There were two pathways for cholesterol esterification in the plasma. One was active in HDL and was blocked almost completely by Intralipid or chylomicron during the lipolysis. The other was active in other lipoproteins and was not inhibited by Intralipid or chylomicron during lipolysis.
Journal
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- The Journal of Japan Atherosclerosis Society
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The Journal of Japan Atherosclerosis Society 14 (2), 447-453, 1986
Japan Atherosclerosis Society
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Details 詳細情報について
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- CRID
- 1571417127937241216
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- NII Article ID
- 130006849781
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- Data Source
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- CiNii Articles