BIOCHEMICAL STUDIES ON INTERMEDIATE METABOLISM OF VITAMINS:I. ON INTERACTION OF VITAMIN B<sub>1</sub> AND SULFHYDRYL GROUP IN PROTEIN MOLECULES

J-STAGE
  • SAHASHI YOSHIKAZU
    Laboratory of Biochemistry, Faculty of Agriculture, University of Tokyo, Tokyo and University of Nagoya
  • FUNAHASHI SABURO
    Laboratory of Biochemistry, Faculty of Agriculture, University of Tokyo, Tokyo and University of Nagoya
  • YAMASHITA KYOHEI
    Laboratory of Biochemistry, Faculty of Agriculture, University of Tokyo, Tokyo and University of Nagoya
  • AKATSUKA TADAMI
    Laboratory of Biochemistry, Faculty of Agriculture, University of Tokyo, Tokyo and University of Nagoya

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1. Interaction of vitamin B1 disulfide and activated sulfhydryl groups in peptides or protein molecules was studied and probable forms of B1-S-S-glutathione, B1-S-S-papain, B1-S-S-eggalbumin and B1-S-S-serum albumin were observed by paper chromatography as shown in Tables I-VI.<br> 2. Interaction of vitamin B1 disulfide and pantetheine also seems to produce B1-S-S-pantetheine.<br> From these findings, probable reaction in intermediate metabolism of vitamin B1 is discussed.<br> The authors wish to express their sincere thanks to Prof. Y. Kuno (Chairman, Committee on Vitamin B Research). The costs of this research have been defrayed from the Department of Education to which the authors' thanks are due.

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