CONTRACTILE PROTEINS FROM ADDUCTORS OF PECTEN:IV. CONSTITUTION OF MYOSIN B

  • YAGI KOICHI
    Chemistry Department, Faculty of Science, Hokkaido University

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1. Four kinds of myosin B of various extraction time-3 or 10 minutes, 30 minutes, 3 hours and 24 hours-were prepared from the fast adductor muscle of Pecten yessoensis.<br> 2. By extension of extraction time from 10 minutes to 30 minutes or to 24 hours, ATP sentitivity increases twice.<br> 3. Composition of four kinds of myosin B was investigated by salting-out analysis, and it was found that they consist mostly of myosin and actomyosin.<br> 4. Their functional unit weights increase slightly with extraction time.<br> 5. From these results it was concluded that the properties of myosin B are changed by the combination of ATP with the active site<br>of the myosin molecule.<br> 6. Mg_??_strengthens the combination of ATP at the active site, and Ca_??_weakens the combination.<br> I wish to thank to Prof. Y. Sakamoto (Department of General Education) and Prof. N. Takasugi (Chemistry Department) for their support; to Assist. Prof. Tono-mura (Research Institute for Catalysis) for his warm encouragement and valuable advice. This study has been aided in part by a grant in aid from the Fundamental Scientific Fund of the Ministry of Education given to the Research Group on the "Chemistry of Muscular Contraction"

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