Biochemical Studies on Sulfate-Reducing Bacteria:X. Adenosine-5'-Phosphosulfate Reductase

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  • ISHIMOTO MAKOTO
    Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo
  • FUJIMOTO DAISABURO
    Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo

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説明

Adenosine-5'-phosphosulfate reductase was purified by 40 times from the extract of Desulfovibrio desulfricans. This preparation catalyzed the reduction of APS to sulfite using methylviologen as an electron carrier. It can also reduce guanosine-5'-phosphosulfate, cytidine-5'-phosphosulfate and uridine-5'-phos-phosulfate. AMP inhibited the APS reduction but SH reagent such as arsenite or p-chloro-mercuribenzoate or metal chelating agents had no effect. Reversibility of APS reductase was also confirmed.<br> The authors thank Prof. F. Egami for his valu-able advice and Dr. Shimojim a for preparation of S35-sulfite. The expences of this work were aided partly by the Scientific Research Funds from the Ministry of Education.

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