Simple Method of Synthesizing Nickel–Nitrilotriacetic Acid Gold Nanoparticles with a Narrow Size Distribution for Protein Labeling

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<jats:p> We developed a simple method to synthesize nickel–nitrilotriacetic acid gold nanoparticles (Ni–NTA Au NPs) with a narrow size distribution for site-specific labeling in protein complexes. Au NPs were synthesized by the reduction of HAuCl<jats:sub>4</jats:sub> using trisodium citrate and tannin acid. Then, the nanoparticle surfaces were modified with NTA and subsequent complexation with Ni<jats:sup>2+</jats:sup>. The mean diameter of the synthesized Ni–NTA Au NPs was 4.3 nm, and the coefficient of variation was 9%. The specific binding of the Ni–NTA Au NPs to polyhistidine-tagged (His-tagged) proteins was determined by transmission electron microscopy using kinesin and the p62 subunit of dynactin. Consequently, our method is useful for analyzing the substructures of protein complexes. </jats:p>

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