Two glycosulfatases from the liver of a marine gastropod, Charonia lampas. Partial purification and properties.:Partial Purification and Properties

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Description

Two glycosulfatases [EC 3.1.6.3], I and II, were purified 31.3- and 33.9-fold respectively, from a crude extract of the liver of Charonia lampas. The purification was carried out by the following chromatographic procedures; phosphocellulose, Sephadex G-150, Concanavalin A-Sepharose and isoelectric focussing. The enzyme preparations obtained were practically free from arylsulfatase [EC 3.1.6.1] contamination. Both glycosulfatases are probably glycoproteins differing in their carbohydrate moieties. The molecular weights of glycosulfatase I and II were estimated to be about 112, 000 and 79, 000, respectively. They had the same optimum pH of 5.5, and the same Km value of 25.0mM for glucose 6-sulfate.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 79 (1), 27-34, 1976

    The Japanese Biochemical Society

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