Intracellular Transfer of Nucleic Acids:I. Fate of Nucleic Acids in Rat Liver Cells

J-STAGE
  • KUSAKARI TAKASHI
    the Department of Physiological Chemistry, Chiba University, School of Medicine
  • YANO MASATA
    the Department of Physiological Chemistry, Chiba University, School of Medicine
  • MITSUHASHI MINORU
    the Department of Physiological Chemistry, Chiba University, School of Medicine
  • NAGATA ICHIRO
    the Department of Physiological Chemistry, Chiba University, School of Medicine
  • OHMURA SHOKO
    the Department of Physiological Chemistry, Chiba University, School of Medicine
  • MIURA YOSHIAKI
    the Department of Physiological Chemistry, Chiba University, School of Medicine

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説明

Wealing rats were injected intraperitoneally with C14-AICA and after 1, 4, 8 and 24 hours, the livers were removed. Nuclear and cytoplasmic ribonucleoproteins were fractionated by DEAE cellulose column chromatography. Cytoplasmic ribonucleoprotein can be divided into four fractions: The supernatant fraction was of nuclear origin and the most metabolically active; the post-microsomal fraction was found radioactive and its specific activity became higher than that of the supernatant fraction, over a period of 4 hours; radioactivities appeared in S-RNA and ribosomes in the later stage.<br> DNA was not wholly incorporated by C14-AICA. DNA, activite in metabolism, was isolated by means of ECTEOLA cellulose column chromatography. Simultaneous administration of purine and pyrimidine precursors resulted in higher incorporation of labeled precursors into the supernatant fraction.<br> This investigation was supported by grants from the National Institute of Health, U. S. A. (CY-5869 CY).

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