Amino acid sequence of a trypsin-chymotrypsin inhibitor, B-III, of peanut (Arachis hypogaea).

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Description

The amino acid sequence of peanut trypsin-chymotrypsin inhibitor, B-111, was determined by conventional methods. The limited proteolysis of B-Ill with trypsin indicated the reactive sites of B-III for trypsin to be Arg(10)-Arg(11) and Arg(38)-Ser(39). Comparison of the established sequence of B-III with those of other Bowman-Birk type double-headed protease inhibitors indicated that B-Ill has four amino acid insertions and one amino acid deletion. It is especially interesting that the amino acid residue in the P1' position (No. 11) of the first reactive site for trypsin is arginine instead of serine, which seems to be conserved at the P, ' position of the reactive sites of all Bowman-Birk type protease inhibitors.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 93 (2), 479-485, 1983

    The Japanese Biochemical Society

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