THE STRUCTURE OF CYTOCHROME C:IV. TREATMENT WITH CARBOXYPEPTIDASE AND AMINOPEPTIDASE

J-STAGE

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Description

More than seven moles of amino acids were liberated from the C-terminal of cytochrome c by the treatment with carboxypeptidase. The catalytic activity of the protein in a succinic oxidase system was retained through out the course of digestion.<br> Leucine aminopeptidase prepared from swine kidney did not split N-terminal amino acid from native cytochrome c.<br> Authors express their thanks to Prof. S. Akabori and Prof. H. Yoshika wa for constant guidances in the course of the work. Thanks are also due to Mr. Y. Nagai and Mr. Tobita of the Institute of Science and Technology, University of Tokyo for the generous gift of leucine aminopeptidase and carboxypeptidase used in the preliminary experiments.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 46 (2), 151-154, 1959

    The Japanese Biochemical Society

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