Effect of Grifolan on the Ascites Form of Sarcoma 180(Biological)

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The antitumor activity of a (1→3)-β-D-glucan, grifolan, which was obtained from a fungus, Grifola frondosa, on the ascites form of Sarcoma 180 in ICR mice was examined. Grifolan waseffective on the ascites tumor when it was administered before tumor transplantation (days-7-1) as a single dose of 2mg/mouse by i.p. injection, but administration after the tumortransplantation was ineffective. The antitumor activity was transferred to untreated mice byinjecting the peritoneal exudate cells obtained from grifolan-treated mice. The antitumor activity ofgrifolan was augmented by vaccination of mice with live sarcoma 180 cells(s.c. injection).

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