Studies on P-450:III. On the Absorption Spectrum of P-450 in Rabbit Liver Microsomes

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1. Liver microsomes were obtained from both phenobarbital-treated and untreated rabbits, and used for the studies on the spectral properties of P450.<br> 2. The absorption spectra of the control and the induced microsomes were measured using, as the reference of spectrophotometry, the microsomes which were decolorized by the hydrogen peroxide-treatment.<br> 3. The spectrum of the induced components was obtained by taking the difference spectrum, induced minus control, with the preparations which were so adjusted as to contain the same amount of cytochrome b5.<br> 4. The approximate absorption spectrum of P-450 in liver microsomes was obtained photometrically by subtracting the spectrum of the preparation, which had been subjected to a prolonged aerobic treatment with pCMB, from the spectrum of the untreated control. The resulting spectrum showed essentially the same characteristics as that of P-450 in adrenal cortex mitochondria.<br> 5. The nature of the colored prosthetic group of P-450 and the limitation in the accuracy of measurement performed in the present study were discussed.

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