Electron Transport Systems of Lung Microsomes and Their Physiological Functions:I. Intracellular Distribution of Oxidative Enzymes in Lung Cells

J-STAGE

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Rabbit lung cells were homogenized in 0.15M KCl containing 10mM phosphate buffer, pH 7.4, and the homogenate was fractionated by differential centrifugation. The distribution patterns of chemical components and oxidative enzymes in lung subcel-lular fractions were similar to those in liver cells. Lung mitochondria (Fraction II) showed a cytochrome pattern characteristic for the mitochondrial respiratory chain, but the contents of cytochromes were only about one fifth those in liver mitochondria. Lung microsomes (Fraction III) contained cytochromes b5 and P-450 as well as NADH-and NADPH-cytochrome c reductase activities. The contents of the cytochromes and the specific activities of the reductases were, however, one third to one fifth the levels reported for liver microsomes. The NADH-reduced minus oxidized differ-ence spectrum of lung microsomes, representing the difference spectrum of cyto-chrome b5, was slightly different from that of liver microsomes.

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