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Parinaric acid as a sensitive fluorescent probe for the determination of lipid peroxidation
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Description
The decrease in fluorescence of conjugated polyenic acyl chains is used as a sensitive assay for lipid peroxidation. The fatty acid cis-trans-trans-cis-9,11,13, 15-octadecatetraenoic acid (cis-parinaric acid) is introduced into liposomal membranes as free fatty acid or, by using the PC specific transfer protein from bovine liver, as 1-palmitoyl-2-cis-parinaroyl-sn-glycero-3-phosphocholine. The peroxidation process as monitored by the decrease in fluorescence intensity is compared with other peroxidation assay systems. Applications of the new assay system are discussed.
Journal
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- Biochim Biophys Acta
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Biochim Biophys Acta 921 266-274, 1987
Elsevier BV
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Keywords
- Diergeneeskunde
- Lipid Peroxides
- Lysophosphatidylcholines
- lipid peroxidation
- Hydrogen Peroxide
- parinaric acid
- (Bovine liver)
- Androgen-Binding Protein
- Spectrometry, Fluorescence
- fluorescent probe
- Liposomes
- Fatty Acids, Unsaturated
- Phosphatidylcholines
- Phospholipid Transfer Proteins
- Carrier Proteins
- phosphatidylcholine
- Copper
- Fluorescent Dyes
Details 詳細情報について
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- CRID
- 1573105976146780416
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- NII Article ID
- 30010391306
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- ISSN
- 00052760
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- PubMed
- 3651488
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- Data Source
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- CiNii Articles
- OpenAIRE