Immunochemical and immunohistochemical localization of Bcl-x protein in the rat central nervous system

  • Mizuguchi Masashi
    Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, NCNP Department of Ultrastructure and Histochemistry Tokyo Institute of Psychiatry
  • Sohma Osamu
    Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, NCNP
  • Takashima Sachio
    Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, NCNP
  • Ikeda Kazuhiko
    Department of Ultrastructure and Histochemistry Tokyo Institute of Psychiatry
  • Yamada Mitsunori
    Department of Pathology, Brain Research Institute, Niigata University
  • Shiraiwa Nobuko
    Department of Biochemistry, Jichi Medical School
  • Ohta Shigeo
    Department of Biochemistry, Jichi Medical School Division of Biochemistry, Institute of Gerontology, Nippon Medical School

この論文をさがす

説明

To explore the role of bcl-x in the regulation of cell death in the nervous system, we produced monoclonal antibodies against rat Bcl-xL protein, the major product of the rat bcl-x gene that inhibits apoptosis, and defined its distribution in rat neural tissues by immunochemical and immunohistochemical means. Western blotting of tissue homogenates identified the Bcl-x protein as two bands with molecular weights of about 29 and 31 kDa. The level of Bcl-x expression in the nervous system was high, being comparable to that in the hematolymphoid system, and higher in the fetal than in the adult brain. Subcellular fractionation studies localized Bcl-x to various subcellular compartments. In tissue culture, Bcl-x was produced by all the cell types examined, including neurons, astrocytes, oligodendrocytes and microglial cells. Immunohistochemistry revealed that Bcl-x immunoreactivity was more intense in the gray than in the white matter. In the fetal cerebral cortex, labeling was mostly confined to the neuronal perikarya, whereas in the more mature brain, the neuropil of the gray matter, as well as the glial cells in the white matter, was also stained.

収録刊行物

キーワード

詳細情報 詳細情報について

  • CRID
    1573105976674194048
  • NII論文ID
    110001801082
  • NII書誌ID
    AN1047681X
  • ISSN
    13409662
  • 本文言語コード
    en
  • データソース種別
    • CiNii Articles

問題の指摘

ページトップへ