バラ'バルカロール'からの体細胞胚形成

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  • FUKUI H.
    FACULTY OF AGRICULTURE GIFU UNIVERSITY, Department of Controlled Plant Production
  • IMAIDA Kazuo
    FACULTY OF AGRICULTURE GIFU UNIVERSITY, Department of Controlled Plant Production

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Other Title
  • Somatic embryogenesis in Rosa hybrida L. cv. Barkarole

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茎頂組織を10.0μM BAPと1.0μM GA3を添加したMS培地で培養した結果,茎頂髄組織あるいは葉原基由来のEmbryogenic callusが誘導された。Embryogenic callusを組織学的に観察した結果,柔組織のなかに球状胚や分裂組織が認められた。Embryogenic callusはショ糖30g/l,BAP 10.0μM,GA3 1.0μM,ゲランガム2.0g/lを添加したMS培地で増殖させることができた。Embryogenic callusからの体細胞胚形成はショ糖60g/l,BAP 10.0μM,GA3 1.0μM,ゲランガム2.0g/lを添加したMS培地で促進された。MS培地にショ糖60g/l,BAP 0.01μM,GA3 10.0μMを添加した液体培地で回転振とう培養した結果,多数の体細胞胚が単離できた。
Embrypgenic callus was derived from the leaf primordia or pith of the shoot tip on a medium containing 10.0μM BAP and 1μM GA3. It had meristematic cells, globular embryos and parenchymas, and was maintained on a medium supplemented with 30 g/l sucrose, 10.0μM BAP, 0.1μM GAS and 2.0 g/l gellangum. Embryogenesis from embryogenic callus was enhanced on a medium containing 60 g/l sucrose, 10.0μM BAP, 1.0μM GAS and 2.0 g/l gellan gum. Many embryos were isolated in a liquid medium containing 0.01μM BAP and 10.0μM GAS which was shaken rotationally at 100 rpm. Cultures 1 to 2 mm in length consisted of individual embryos; those over 2 mm in length consisted of a mass of embryos able to divide into individual embryos without difficulty.

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Details 詳細情報について

  • CRID
    1573105976675171072
  • NII Article ID
    110001000874
  • NII Book ID
    AN00053423
  • ISSN
    00724513
  • Text Lang
    en
  • Data Source
    • CiNii Articles

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