Detection of Histidine Decarboxylase (HDC) in Paraffin Sections of Formalin Fixed Rat Stomach Using Avidin-biotin-peroxidase-complex Method
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- MURAKOSHI Masanori
- Safety Research Department, Teikoku Hormone Mfg. Co. Ltd., Kawasaki Department of Pathology, Tokai University School of Medicine,
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- INADA Rie
- Safety Research Department, Teikoku Hormone Mfg. Co. Ltd., Kawasaki
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- SUZUKI R. Minoru
- Safety Research Department, Teikoku Hormone Mfg. Co. Ltd., Kawasaki
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- WATANABE Keiichi
- Department of Pathology, Tokai University School of Medicine
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- TAGUCHI Yoshitaka
- Department of Pharmacology II, Osaka University, School of Medicine, Osaka
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- WADA Hiroshi
- Department of Pharmacology II, Osaka University, School of Medicine, Osaka
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説明
Immunohistochemical localization of histidine decarboxylase (HDC) in rat stomach was studied. Formalin fixed, paraffin sections were prepared and immunohistochemical localization of HDC was performed by the avidin-biotin-peroxidase-complex method (ABC method). As a result, HDC was clearly detected in the basal portion of the oxyntic glands. The shape of many HDC-positive cells were flasklike. No reaction products were seen in chief cells or parietal cells. These findings are in agreement with the results reported by many workers. Therefore, ABC method of HDC is thought to be a useful technique for the detection of enterochromaffin-like cells using paraffin sections.
収録刊行物
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- Tokai journal of experimental and clinical medicine
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Tokai journal of experimental and clinical medicine 12 (3), 197-199, 1987-09
東海大学
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詳細情報 詳細情報について
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- CRID
- 1573105976804678528
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- NII論文ID
- 110004700414
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- NII書誌ID
- AA00863975
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- ISSN
- 03850005
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles