- 【Updated on May 12, 2025】 Integration of CiNii Dissertations and CiNii Books into CiNii Research
- Trial version of CiNii Research Knowledge Graph Search feature is available on CiNii Labs
- 【Updated on June 30, 2025】Suspension and deletion of data provided by Nikkei BP
- Regarding the recording of “Research Data” and “Evidence Data”
Developmental changes of .GAMMA.-aminobutyric acid (GABA) and putative amino acid neurotransmitters in the organotypic culture of newborn mouse cerebellum.
-
- SATOMI Daisaku
- Department of Biology, College of Arts and Sciences, The University of Tokyo
Description
The quantitative changes and metabolism of GABA and putative amino acid neurotransmitters during early developmental stages in the organotypic culture of newborn mouse cerebellum were examined by using the high-performance liquid chromatograph (HPLC) technique. D-[U-14C]Glucose was used as a precursor of amino acids.<br> To analyze amino acid neurotransmitters, explants were incubated for 4 weeks under standard conditions. The amount of GABA linearly increased from 8.7±1.3nmol/mg protein (2days in vitro, 2 DIV) to 26.5±6.1nmol/mg protein (15 DIV) and was saturated after that (24.0±3.6nmol/mg protein at 30 DIV). During the period of GABA increase, the capability for GABA synthesis from [14C]glucose increased rapidly from 3.03±0.67nCi/mg protein (2 DIV, 3h incubation) to 9.32±1.34nCi/mg protein (15 DIV, 3h incubation).<br> In the case of glutamic acid, a putative neurotransmitter of granule cell parallel fibers in the cerebellum, the amount in explants was nearly constant during incubation, in contrast with the fact that the amount in vivo gradually increased. However, the capability for glutamic acid synthesis from [14C]glucose increased from 10.80±3.01nCi/mg protein (2 DIV, 1h incubation) to 27.62±4.71nCi/mg protein (22 DIV, 1h incubation). In the case of taurine, found in abundance in fetal brain and supposed to play a specific role in the development and maturation of the central nervous system, the amount in explants decreased from 139.8±4.0nmol/mg protein (2 DIV) to 54.0±0.8nmol/mg protein (30 DIV). The pattern of change of taurine was similar to that observed in vivo.<br> On considering together the results obtained in this report and the previous one (Satomi, D. (1983) J. Biochem. 94, 785-791), it was presumed that in the organotypic culture of newborn mouse cerebellum, biochemical changes of neurotransmitters are closely related to morphological development in nearly the same manner as in vivo.
Journal
-
- The Journal of Biochemistry
-
The Journal of Biochemistry 100 (2), 285-292, 1986
The Japanese Biochemical Society
- Tweet
Details 詳細情報について
-
- CRID
- 1573105977801184512
-
- NII Article ID
- 130006874893
-
- Text Lang
- en
-
- Data Source
-
- CiNii Articles