The biosynthesis of transfer RNA in insects. II. Isolation of transfer RNA precursors from the posterior silk gland of Bombyx mori.:II. Isolation of Transfer RNA Precursors from the Posterior Silk Gland of Bombyx mori

TSUTSUMI Ken-ichi, MAJIMA Reiko, SHIMURA Kensuke

The biosynthesis of transfer RNA in insects. II. Isolation of transfer RNA precursors from the posterior silk gland of Bombyx mori.:II. Isolation of Transfer RNA Precursors from the Posterior Silk Gland of Bombyx mori

J-STAGE

TSUTSUMI Ken-ichi

Laboratory of Biochemistry, Department of Agricultural Chemistry, Tohoku University
MAJIMA Reiko

Laboratory of Biochemistry, Department of Agricultural Chemistry, Tohoku University
SHIMURA Kensuke

Laboratory of Biochemistry, Department of Agricultural Chemistry, Tohoku University

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説明

The occurrence of precursors to tRNA in the post-polysomal fraction of the posterior silk gland of Bombyx mori was demonstrated by pulse-chase labeling and DNA-RNA hybridization competition experiments. These precursors had molecular sizes ranging from 4S to 5S on polyacrylamide gel electrophoresis. Analysis of the incorporation of the methyl group from [methyl-¹⁴C] methionine revealed that a radioactive peak on polyacrylamide gel appeared in the 4, 5S region during brief labeling. This suggested that some methylation occurred at the 4.5S precursor step.