The biosynthesis of transfer RNA in insects. II. Isolation of transfer RNA precursors from the posterior silk gland of Bombyx mori.:II. Isolation of Transfer RNA Precursors from the Posterior Silk Gland of <i>Bombyx mori</i>

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  • TSUTSUMI Ken-ichi
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Tohoku University
  • MAJIMA Reiko
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Tohoku University
  • SHIMURA Kensuke
    Laboratory of Biochemistry, Department of Agricultural Chemistry, Tohoku University

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Description

The occurrence of precursors to tRNA in the post-polysomal fraction of the posterior silk gland of Bombyx mori was demonstrated by pulse-chase labeling and DNA-RNA hybridization competition experiments. These precursors had molecular sizes ranging from 4S to 5S on polyacrylamide gel electrophoresis.<br> Analysis of the incorporation of the methyl group from [methyl-14C] methionine revealed that a radioactive peak on polyacrylamide gel appeared in the 4, 5S region during brief labeling. This suggested that some methylation occurred at the 4.5S precursor step.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 80 (5), 1039-1045, 1976

    The Japanese Biochemical Society

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