Studies on the Microsomal Electron-transport System of Anaerobically Grown Yeast:I. Intracellular Localization and Characterization

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The cytochromes of anaerobically grown yeast cells were studied; cytochrome b5 and P-450 were recovered in the fraction which was precipitated between 10, 000×g and 105, 000×g and were identified as yeast microsomes by chemical analysis and electron microscopic examination. These cytochromes from the microsomes were reduced by either NADH or NADPH and the reduced cytochromes were readily oxidized by air. Moreover, evidence for the presence of NADH-cytochrome b5 reductase and NADPH-cytochrome c reductase was obtained.<br> From these results, it was concluded that microsomes of anaerobically grown yeast cells contain the same electron-transferring components as the microsomes of mammalian hepatocytes, i.e., cytochrome b5, P-450, NADH-cytochrome b5 reductase [EC 1. 6. 2. 2] and NADPH-cytochrome c reductase [EC 1. 6. 2. 4]. These components may comprise an electron-transport system resembling that of hepatic microsomes.

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