Effects of Triton X-100 on gel electrophoresis and gel chromatography of histones. Possible binding to helical regions.:Possible Binding to Helical Regions

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  • HAMANA Koei
    Department of Protein Chemistry, Institute of Endocrinology, Gunma University
  • IWAI Koichi
    Department of Protein Chemistry, Institute of Endocrinology, Gunma University

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Description

Polyacrylamide gel electrophoresis of whole histones of calf thymus, chicken erythro-cytes, and Tetrahymena was carried out in the absence or presence of a nonionic surfactant, Triton X-100 (up to 6mM), in 0.9M acetic acid and 6.25M urea-0.9M acetic acid. Calf thymus whole histone was also chromatographed on Bio-Gel P-200, in the absence or presence of 6mM Triton in 0.01M HCl and 8M urea-0.9M acetic acid. Triton reduced the electrophoretic mobility and distribution coefficient of various histone species in the following order of decreasing effect; H2A>H3, H4>H2B>Hl in the absence of urea. Hl and specific histones for chicken erythrocytes and Tetra-hymena were almost unaffected. Urea antagonized the surfactant effect more for H4 and H2B, and less for H2A and H3. Such surfactant effects can be correlated with the helical contents of histone species under the experimental conditions used, rather than their total hydrophobicites, suggesting that Triton binds to helical regions.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 79 (1), 125-129, 1976

    The Japanese Biochemical Society

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