SOME STUDIES ON ISOLATED LIVER CELLS:I. On the durability of the vitality of isolated liver cells and their enzymes

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1. The isolated liver cells retain their nucleus staining capacity for a week. The nucleus-staining ability seems then to begin to decrease. On the 15th day all nuclei are entirely unstained.<br> 2. The activity of peptidase autolytic enzymes, esterase and amylase is retained very well in the preservation of isolated liver cells. The amylase was found most stable.<br> 3. A part of esterase activity fades away in a relatively short time of preservation, while the remaining activity is quite stable.<br> 4. The activity of hvdrolases within the isolated cells is independent of the ability of nucleus to be stained.<br> 5. It was impossible to find the activity of aminoacidase in the isolated liver cells.

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