Amino Acid Sequence of a Human Kappa Type Bence-Jones Protein:I. Tryptic Peptides of Protein Ni

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Description

To determine the amino acid sequence of the human κ Bence-Jones Protein Ni, the protein was completely reduced and aminoethylated and then digested with trypsin [EC 3. 4. 4. 4]. Twenty-five major tryptic peptides were isolated by chromatography on Dowex 1-×2. The complete sequences of 18 of these peptides were determined. These comprised the entire variable NH2-terminal half and 7 peptides from the COOH-terminal half of the protein. Only the amino acid compositions and partial sequences of the other peptides were determined. Only one of the tryptic peptides in the NH2-terminal half of the molecule had the same sequence as that reported for the corresponding region of other human κ chains, whereas all the tryptic peptides covering the sequence from residue 108 to the COOH-terminus corresponded exactly to the sequences reported for the constant region of human κ chains.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 73 (2), 417-431, 1973

    The Japanese Biochemical Society

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