Probing the Secondary Structure of MCS4 RNA of Mycoplasma capricolm.

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  • Ushida Chisato
    Department of Biology, Faculty of Science, Hirosaki University
  • Sasamura Shinpei
    Department of Biology, Faculty of Science, Hirosaki University
  • Muto Akira
    Department of Biology, Faculty of Science, Hirosaki University

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説明

MCS4 RNA is one of the small stable RNAs found in Mycoplasma capricolum. Its function is unknown. The conformation of MCS4 RNA (125 nucleotides in length) in solution was investigated using chemical and enzymatic probes. Single and double-stranded regions were estimated by means of diethyl pyrocarbonate (DEPC) and by dimethyl sulfate (DMS) modifications. Ribonuclease V1 was also used to identify paired or stacked nucleotides. Based on these data, a secondary structure model for MCS4 RNA containing four stemstructures was constructed.

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