Oxidative Phosphorylation Coupled with Nitrate Respiration:I. Evidence for Phosphorylation Coupled with Nitrate Reduction in a Cell-free Extract of <i>Pseudomonas aeruginosa</i>

YAMANAKA TATEO, OTA AKIHIRO, OKUNUKI KAZUO

Oxidative Phosphorylation Coupled with Nitrate Respiration:I. Evidence for Phosphorylation Coupled with Nitrate Reduction in a Cell-free Extract of <i>Pseudomonas aeruginosa</i>

J-STAGE

YAMANAKA TATEO

Department of Biology, Faculty of Science, University of Osaka
OTA AKIHIRO

Department of Biology, Faculty of Science, University of Osaka
OKUNUKI KAZUO

Department of Biology, Faculty of Science, University of Osaka

この論文をさがす

CiNii Books

説明

The oxidative phosphorylation coupled with anaerobic reduction of nitrate was demonstrated in cell-free extracts of P. aeruginosa. The P/NO₃- ratio was about 0.3, with lactate as the substrate. The phosphorylation activity of the cell-free extracts was inactivated by heat treatment, and sensitive to potassium cyanide and DNP. It was presented that the phosphorylation activity required the combination of the particles and the supernatant which were prepared by the centrifugation at 138, 000×g of the cell-free extracts.