Amino Acid Sequence of the Coat Protein of the RNA Phage ZR

J-STAGE

書誌事項

公開日
1970
公開者
The Japanese Biochemical Society

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説明

Amino acid sequence of the coat protein from RNA phage ZR (Group I) was partially determined. Oligopeptides in the tryptic digest from carboxymethylated coat protein were separated on columns of Dowex 50-X2 (soluble fraction at pH 3.1) and Sephadex G-50 (insoluble fraction at pH 3.1). The sum of the amino acids in the tryptic peptides accounts for all of the 129 amino acids present in the coat protein of ZR phage. Of the tryptic peptides, T1, T2, Tl0 and T11 were further digested by other proteolytic enzymes, and resulting smaller peptides were fractionated. Amino acid and terminal analyses were performed with each peptide and amino acid sequences of some of these peptides were determined.<br> The amino acid sequence of the coat protein from ZR phage is proposed in comparison with the primary structures of phage coat proteins belonging to the Group I. No difference between amino acid sequence of the coat protein from ZR phage and those from bacteriophages R17 and MS2 seemed to be present but ZR phage coat protein differs from that of f 2 bacteriophage by a single amino acid substitution.

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