HybridomaとAtherosclerosis (第1報)

J-STAGE
  • 塚田 豊弘
    Department of Internal Medicine, Tokyo Medical and Dental University
  • 広瀬 進
    Department of Internal Medicine, Tokyo Medical and Dental University
  • 黒岩 トモエ
    Department of Internal Medicine, Tokyo Medical and Dental University
  • 矢島 途好
    Department of Internal Medicine, Tokyo Medical and Dental University
  • 沼野 藤夫
    Department of Internal Medicine, Tokyo Medical and Dental University
  • LEE K. T.
    Department of Pathology, Albany Medical College
  • 筒井 正人
    Department of Dermatology, University of Miami
  • 安達 健二
    Department of Dermatology, University of Miami

書誌事項

タイトル別名
  • Hybridoma and Atherosclerosis

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抄録

As the role of macrophage-derived foam cells seems to relate to the progression and regression of atherosclerosis, we attempted to acquire monoclonal antibodies of macrophages, using a hybridoma technique. Use of these antibodies enables identification of macrophage-derived foam cells in atherosclerotic lesions.<br>Using BALB/C and SP2/O mice, we established several clones of mouse hybridoma, which produce monoclonal antibodies against pig alveolar macrophages. #75 antibody obtained in this series was confirmed to be specific for macrophages. With this antibody, we studied atherosclerotic lesions in aorta of pigs fed a one percent cholesterol diet for 20 weeks, using an indirect immunoperoxidase procedure. Numerous foam cells located in fibrous caps or immediately under the fibrous cap as well as the necrotic foci exhibited a positive reaction.<br>Thus, the use of hybri-clonal antibody to determine the role of macrophage-derived foam cells in atherosclerotic lesions should yield pertinent data related to the lesions seen in atherosclerosis and related disorders.

収録刊行物

  • 動脈硬化

    動脈硬化 11 (2), 449-456, 1983

    Japan Atherosclerosis Society

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