Pretreatment with Antioxidants Decreases the Effects of Salt Stress on Chloroplast Ultrastructure in Rice Leaf Segments (Oryza sativa L.)
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- KAWASAKI Michio
- Graduate School of Bioagricultural Sciences, Nagoya University
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- TANIGUCHI Mitsutaka
- Graduate School of Bioagricultural Sciences, Nagoya University
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- MIYAKE Hiroshi
- Graduate School of Bioagricultural Sciences, Nagoya University
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- RAHMAN Shahidur
- Department of Crop Botany, Bangladesh Agricultural University
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- YAMANE Koji
- Graduate School of Bioagricultural Sciences, Nagoya University
書誌事項
- 公開日
- 2004-09-01
- 公開者
- 日本作物学会
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説明
We investigated the kinds of active oxygen species leading to the destruction of chloroplast ultrastructure in salt-stressed rice plants. After the seedlings were grown for 3 wks, leaf segments (5 mm square) were cut from the middle portion of the 5th leaves. Leaf segments were incubated in 200 mM NaCl under dark or light conditions for 24 hr. The chlorophyll content in the leaf segments drastically decreased in light between 12- and 24 hr in 200 mM NaCl, but, no reduction was observed in the dark. In electron microscopic studies, 200 mM NaCl caused swelling of thylakoids and destruction of thylakoid membranes in light. On the other hand, no ultrastructural changes were observed under dark condition. In one experiment, leaf segments were incubated in 200 mM NaCl for 24 hr in light after preincubation with antioxidants for 12 hr in light. Pretreatment with ascorbate and benzoate, which scavenge H_2O_2 and ・ OH, respectively, effectively suppressed the reduction of chlorophyll content and the destruction of chloroplasts by NaCl in light. However, Tiron and DABCO, which scavenge O_2 and ^1O_2, respectively, could not suppress the effects of salt stress in light. Fe-SOD activity was increased about eight time by salt stress (200 mM NaCl), but, catalase activity was reduced to 69% of the control and ascorbate peroxidase activity was not affected by NaCl. These results suggested that salt-induced injury in chloroplasts is dependent on light, and that H_2O_2 and ・ OH are responsible for the deleterious effects of salt stress on chlorophyll content and chloroplast ultrastructure.
収録刊行物
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- Plant production science
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Plant production science 7 (3), 292-300, 2004-09-01
日本作物学会
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詳細情報 詳細情報について
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- CRID
- 1573387451723833728
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- NII論文ID
- 110001720545
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- NII書誌ID
- AA11162156
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- ISSN
- 1343943X
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles