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Temperature-jump studies on benzeneboronic acid-serine protease interactions. Subtilisin and .ALPHA.-chymotrypsin.:Subtilisin and α-Chymotrypsin
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- NAKATANI Hiroshi
- Department of Food Science and Technology, Faculty of Agriculture
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- HANAI Kazumitsu
- Department of Food Science and Technology, Faculty of Agriculture
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- UEHARA Yukiko
- Department of Food Science and Technology, Faculty of Agriculture
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- HIROMI Keitaro
- Department of Food Science and Technology, Faculty of Agriculture
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Description
The interactions of benzeneboronic acid (BBA) as a transition state analog with subtilisin [EC 3. 4. 21. 4] and with α-chymotrypsin [EC 3. 4. 21. 1] were investigated kinetically by the temperature-jump method using pH indicators. For both enzymes, the concentration dependence of the relaxation time was consistent with a two-step mechanism involving a fast bimolecular association followed by a slow, unimolecular process. The possibility of a trigonal-tetrahedral interconversion of BBA at the active site of the enzyme is discussed.
Journal
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- J Biochem (Tokyo)
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J Biochem (Tokyo) 77 (4), 905-908, 1975
The Japanese Biochemical Society
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Details 詳細情報について
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- CRID
- 1573387452939850624
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- NII Article ID
- 130003539634
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- ISSN
- 0021924X
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- Text Lang
- en
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- Data Source
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- CiNii Articles
