STUDIES ON THE BIOSYNTHESIS OF SILK FIBROIN:I. INCORPORATION OF GLYCINE-L-C<sup>14</sup> INTO FIBROIN IN VIVO

J-STAGE
  • SHIMURA KENSUKE
    Laboratory of Biochemistry, Department of Agriculture, Tohoku University
  • FUKAI HIRONOBU
    Laboratory of Biochemistry, Department of Agriculture, Tohoku University
  • SUTO SEIICHI
    Laboratory of Biochemistry, Department of Agriculture, Tohoku University
  • HOSHI REIKO
    Laboratory of Biochemistry, Department of Agriculture, Tohoku University

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Description

1. A large part of glycine-l-C14 injected into the body cavity of silkworm was incorporated into the posterior silk-gland.<br> 2. Two methods (Griffin' s and TCA method) were attempted to fractionate the proteins of the posterior silk-gland. The change of specific radioactivity of fibroin prepared by TCA method showed a lag phase during first 30 minutes after the injection.<br> 3. The large granule of the cells of the posterior silk-gland incorporated glycine-l-C14 more than the small granule.<br> 4. Location of radioactivity in amino acids of fibroin was determined by a method of Dowex-50 column chromatography. About 95 per cent of the total radioactivity recovered was shown to be located in glycine residue.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 45 (7), 481-488, 1958

    The Japanese Biochemical Society

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