Achromobacter cycloclastes nitrite reductase. The function of copper, amino acid composition, and ESR spectra.:The Function of Copper, Amino Acid Composition, and ESR Spectra

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1. Dialysis against cyanide at pH 7 of Achromobader cydoclastes nitrite reductase [EC 1. 7. 99. 3] of a dissimilatory type led to the removal of about 50% of the copper from the enzyme molecule, with a concomitant decrease of the enzymatic activities. It was inferred that enzyme-bound copper atoms play an essential role in the catalytic activities of the enzyme.<br>2. The amino acid composition of the enzyme was determined after acid hydrolysis.<br>3. ESR spectra of the frozen solution and lyophilized powder of the nitrite reductase predominantly showed the presence of two kinds of copper: Type 1 Cu2+, which had narrow and sharp hyperfine splitting, and Type 2 Cu2+, which had broader hyperfine splitting. The bond between the oxidized enzyme and nitrite seems to be ionic.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 78 (2), 355-361, 1975

    The Japanese Biochemical Society

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