Studies on the Toxic Substances Isolated from Mycobacteria:II. Toxic Glycolipids of <i>Mycobacterium smegmatis, Mycobacterium phlei</i> and Atypical Mycobacteria Strain <i>No. 6</i> and <i>No. 22</i>

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  • AZUMA ICHIRO
    Department of Biochemistry, School of Medicine, Kyushu University, Fukuoka, and Research Laboratory of the National Sanatorium
  • NAGASUGA TOKUKO
    Department of Biochemistry, School of Medicine, Kyushu University, Fukuoka, and Research Laboratory of the National Sanatorium
  • YAMAMURA YUICHI
    Department of Biochemistry, School of Medicine, Kyushu University, Fukuoka, and Research Laboratory of the National Sanatorium

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Description

1. Purified waxes were extracted from Mycobacterium smegmatis, Mycobacterium phlei and atypical mycobacteria strain No. 6 and No. 22 in the yields of 2 to 4 per cent of bacilli.<br> 2. Toxic lipids were fractionated from purified wax by column chromatographies on silica gel, silicic acid and Florisil and their yields were 0.3 to 2.2 per cent of purified wax.<br> 3. These glycolipids were active at 0.01mg. dose levels in the toxicity to mice.<br> 4. Lipid moiety of these glycolipids were mycolic acids. Mycolic acids of Mycobacterium smegmatis and Mycobacterium phleiii were different from mycolic acid of tubercle bacilli and assumed to have the formula C67H134O6.<br> 5. Water soluble fraction of these toxic glycolipids was trehalose.<br> The authors express their deep gratitude to Dr. H. Oura, School of Pharmacy, Toyama University, for his helpful suggestion and Dr. A. Yasuda and Dr. Y. Fujiwara for cultivation of bacilli. The authors are indebted to Mrs. S. Matsuba for elementary analysis, and to Mr. K. Hikita for spectral measurement.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 52 (2), 92-98, 1962

    The Japanese Biochemical Society

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