PHOSPHORUS METABOLISM OF FUNGAL CELLS:II. SOME PROPERTIES OF THE OXIDATIVE PHOSPHORYLATION SYSTEM OF MITOCHONDRIA FROM ASPERGILLUS ORYZAE

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  • IWASA KOZO
    Department of Biology, Faculty of Science, University of Osaka

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Description

Some properties of mitochondria from Aspergillus oryzae were studied. Only succinate dehydrogenase activity remained. Other dehydrogenases such as isocitric, lactic, pyruvic, α-ketoglutaric, β-hydroxybutyric, and the reduced DPN-oxidizing enzyme system were lost during preparation of the mito-chondria.<br> Cytochrome c and serum albumin prevented loss of activity to a certain extent. A nucleotide complex prepared from the fungal mats prevented aging of the mitochondria. The active materials in this preparation are nucleotides commonly found in the cells. Stimulation of phosphorylation was also observed, but the action mechanism is still obscure.<br> The author wishes to express his thanks to Prof. Okunuki for his valuable guidance. Thanks are due to his colleagues Mr. T. Higashiyama of the same laboratory for their help and discussion, and Dr. Inagaki of the same laboratory for kindly supplying glutamic dehydrogenase.

Journal

  • J Biochem (Tokyo)

    J Biochem (Tokyo) 47 (5), 584-591, 1960

    The Japanese Biochemical Society

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