Reversion to Bacillary Forms of <i>Serratia marcescens</i> Spheroplasts Induced by Carbenicillin

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  • FURUTANI Akio
    Department of Biology, Osaka Shin-ai Jogakuin Junior College|Department of Bacteriology, Kinki University School of Medicine
  • TADA Yoshifumi
    Department of Bacteriology, Kinki University School of Medicine
  • YAMAGUCHI Junji
    Department of Bacteriology, Kinki University School of Medicine

説明

Bacterial cells of Serratia marcescens were easily induced to form spheroplasts in liquid medium by the addition of carbenicillin. The spheroplasts were unable to divide, but they were able to revert to the bacillary forms in liquid medium not containing carbenicillin. Four phases of the reversion sequence could be differentiated by scanning electron microscopy. (1) After 3hr of incubation in carbenicillin-free medium, some projections arose out of the spheroplasts, and grew and elongated. (2) Their elongation resulted in a morphological change in the spheroplasts from spherical bodies to long irregular bacillary forms. (3) Further incubation caused several constricted areas in the bacillary form. (4) The long bacillary forms split along the constricted areas to become the parent bacillary forms of S. marcescens. When the long bacillary form that developed during the reversion was retreated with carbenicillin, it was immediately induced to become a spheroplast again.

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