超遠心法による血清リポ蛋白VLDL, LDLおよびHDLコレステロールの健常者平均値

We have developed a techinique to fractionate serum lipoproteins with a use of Beckman Lp-42 Ti rotor. By ultracentrifugation at 42, 000 rpm, 222, 000 G, at 8°C, for 150 minutes, this rotor separates 36 pairs of serum samples, 175μl each in two cellulose propionate tubes for one subject, into VLDL and LDL+HDL fractions in a tube of the background density at 1.006, and VLDL+HDL and HDL fractions in another tube of background density at 1.063. Cholesterol concentrations are determined for total cholesterol (TC), LDL+HDL and HDL fractions. By substraction TC, VLDLc=TC-(LDL+HDL), LDLc=(LDL+HDL)-HDL, and HDLc are obtained.<br>Two hundreds eighty nine subjects with an average age of 46±16 years, 148 male (50±14years) and 141 female (42±17 years), who visited Keio Health Counselling Center for their health check and were found without any serious abnormalities in their clinical and laboratory tests, were examined for their fasting serum cholesterol, triglycerides and serum lipoprotein cholesterol levels fractionated by using the rotor.<br>Total cholesterol averaged 202±35mg/dl (204±36mg/dl for male, 200±34mg/dl for female), triglycerides 109±61mg/dl (male 133±72mg/dl. female 91±44mg/dl), VLDL cholesterol 17±21mg/dl (male 22±21mg/dl, female 13±19mg/dl), LDL cholesterol 135±27mg/dl (male 136±27mg/dl, female 134±27mg/dl) and HDL cholesterol 51±11mg/dl (male 48±10mg/dl, female 54±11mg/dl). The ratio of total cholesterol minus HDL cholesterol/HDL cholesterol, and LDL-cholesterol/HDL cholesterol were calculated as 3.2+ 1.1 (male 3.4+ 1.1, female 2.9+ 1.1) and 2.8±0.9 (male 2.9±0.9, female 2.6±0.8), respectively.<br>These results indicate that, though they are still lower than those of Western countries, they tend to be higher when compared with the cholesterol values measured in a general population of this country in 1960 and 1970.