Overproduction of Cellular and Activated Ha-ras Proteins by Mutating a Synthetic Gene(Biological,Chemical)
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- 三浦 一伸
- Faculty of Pharmaceutical Sciences, Hokkaido University
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- 紙谷 浩之
- Faculty of Pharmaceutical Sciences, Hokkaido University
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- 富永 峰子
- Faculty of Pharmaceutical Sciences, Hokkaido University
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- 井上 泰彦
- Faculty of Pharmaceutical Sciences, Hokkaido University
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- 池原 森男
- Faculty of Pharmaceutical.Sciences, Osaka University
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- 野口 茂
- National Cancer Center Research Institute
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- 西村 進
- National Cancer Center Research Institute
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- 大塚 栄子
- Faculty of Pharmaceutical Sciences, Hokkaido University
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説明
A synthetic gene for c-Ha-ras (Vat-12) has been modified by cassette mutagenesis using restriction sites, Cla1-BssHII, to encode c-Ha-ras Gly-12. Genes for c-Ha-ras (Leu-61 and Arg-61) have been synthesized by joining newly synthesized oligodeoxyribonucleotides containing appropriate codons, together with previously obtained synthetic fragments. These genes have been expressed in E. coli and the products (p21) isolated. The guanosine diphosphate binding properties and guanosine triphosphatase activities of these p21 derivatives were studied.
収録刊行物
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- Chem. Pharm. Bull.
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Chem. Pharm. Bull. 35 4878-4882, 1987
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詳細情報 詳細情報について
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- CRID
- 1573668927091920896
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- NII論文ID
- 110006281740
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- NII書誌ID
- AA00602100
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- ISSN
- 00092363
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles